{"id":18094,"date":"2022-12-22T13:21:49","date_gmt":"2022-12-22T13:21:49","guid":{"rendered":"https:\/\/iiser.ntc-us.com\/iisernew\/?p=18094"},"modified":"2023-02-03T07:33:22","modified_gmt":"2023-02-03T07:33:22","slug":"conversion-of-a-defensive-toxin-antitoxin-system-into-an-offensive-t6ss-effector-in-burkholderia","status":"publish","type":"post","link":"https:\/\/iiser.ntc-us.com\/iisernew\/conversion-of-a-defensive-toxin-antitoxin-system-into-an-offensive-t6ss-effector-in-burkholderia\/","title":{"rendered":"Conversion of a defensive toxin-antitoxin system into an offensive T6SS effector in Burkholderia"},"content":{"rendered":"

Title<\/strong> : Conversion of a defensive toxin-antitoxin system into an offensive T6SS effector in Burkholderia.
\nSpeaker<\/strong> : Dr Gopaljee Jha, Staff Scientist V, Plant-Microbe Interactions Lab, NIPGR, New Delhi.
\nDate<\/strong> : 09\/11\/2021, 06:00 PM (Monday), Online Mode.<\/p>\n

Abstract:<\/u><\/strong><\/p>\n

We have previously identified\u00a0Burkholderia\u00a0gladioli<\/em> strain NGJ1 from rice, which exhibits\u00a0a\u00a0novel ability to feed on fungi,\u00a0a\u00a0phenomenon known as mycophagy. We have demonstrated that functional type III secretion\u00a0system\u00a0(T3SS) is required for microphagous ability\u00a0of\u00a0NGJ1. Further, we have reported that NGJ1 deploys\u00a0a\u00a0prophage tail-like protein (Bg_9562)\u00a0into\u00a0fungal cells\u00a0in\u00a0a\u00a0T3SS dependent manner to feed on fungi. Our recent study has revealed that beside mycophagy, NGJ1 also exhibits broad-spectrum antibacterial activity and type VI secretion\u00a0system\u00a0(T6SS) is required for contact-dependent killing\u00a0of\u00a0the prey bacteria. Generally,\u00a0bacteria utilize various kinds\u00a0of\u00a0toxins to either inhibit the growth\u00a0of\u00a0co-habiting bacteria or when needed control their own growth. We report that\u00a0Burkholderia<\/em>\u00a0and certain other bacteria have altered the potential\u00a0defensive\u00a0function\u00a0of\u00a0Tox-REase-5 domain containing toxins\u00a0into\u00a0offensive\u00a0function. The NGJ1 encodes such toxins as\u00a0T6SS\u00a0effectors (TseTBg) and potentially deploys them to kill co-habiting rice endophytic bacteria. Notably, the immunity (TsiTBg) proteins associated with TseTBg effectors demonstrate functional similarity with the antitoxin\u00a0of\u00a0type II\u00a0toxin-antitoxin\u00a0(TA)\u00a0system. Genome analysis\u00a0of\u00a0diverse bacteria revealed that various TseTBg orthologs are either encoded as TA or\u00a0T6SS\u00a0effectors.\u00a0In\u00a0addition, potential evolutionary events associated with\u00a0conversion\u00a0of\u00a0TA\u00a0into\u00a0T6SS\u00a0effectors have been delineated. Our results indicate that the transposition\u00a0of\u00a0IS3 elements has led to the operonic fusion\u00a0of\u00a0certain\u00a0T6SS\u00a0related genes with TA genes resulting\u00a0in\u00a0their\u00a0conversion\u00a0into\u00a0T6SS\u00a0effectors. Such\u00a0a\u00a0genetic change has enabled bacteria to utilize novel toxins to precisely target co-habiting bacteria.<\/p>\n

 <\/p>\n

Bio sketch<\/u><\/b><\/span>:<\/u><\/strong><\/p>\n

Gopaljee Jha\u00a0obtained his M.Sc\u00a0in\u00a0Biotechnology from Guru Nanak Dev University, India. He has completed his PhD (in\u00a02008) from CSIR-CCMB, Hyderabad, under the supervision\u00a0of\u00a0Dr Ramesh Sonti. Then, he joined as\u00a0a\u00a0scientist at CSIR-Institute\u00a0of\u00a0Himalayan Bioresource Technology, Palampur, H.P, India. He carried out his postdoctoral research at Sainsbury Laboratory, John Innes Centre, Norwich, U.K. He is currently working as Staff Scientist V\u00a0in\u00a0the Plant-Microbe Interactions unit\u00a0of\u00a0the National Institute\u00a0of\u00a0Plant Genome Research, New Delhi. His group carries integrated research with the main focus on understanding how pathogens cause disease and plants defend themselves. He is\u00a0a\u00a0recipient\u00a0of\u00a0the INSA medal for Young Scientist (2010), NASI Platinum Jubilee Young Scientist Award (2007), BOYSCAST Fellowship from the Department\u00a0of\u00a0Science and Technology (2010-2011) and DST-Swarna Jayanti Fellowship 2020.<\/p>\n","protected":false},"excerpt":{"rendered":"

Date<\/strong> : 09\/11\/2021, 06:00 PM (Monday)<\/p>\n","protected":false},"author":8,"featured_media":21956,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_expiration-date-status":"","_expiration-date":0,"_expiration-date-type":"","_expiration-date-categories":[],"_expiration-date-options":[]},"categories":[109],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/posts\/18094"}],"collection":[{"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/comments?post=18094"}],"version-history":[{"count":6,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/posts\/18094\/revisions"}],"predecessor-version":[{"id":23213,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/posts\/18094\/revisions\/23213"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/media\/21956"}],"wp:attachment":[{"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/media?parent=18094"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/categories?post=18094"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/iiser.ntc-us.com\/iisernew\/wp-json\/wp\/v2\/tags?post=18094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}